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SlideshowReport

Model for the events occurring around a single replication fork

Description
The replication fork is generated when helicase untwists the DNA to produce two single-stranded template
strands. The process of separation of double-stranded
DNA to two single strands is called DNA denaturation
or DNA melting. Single-strand DNA-binding (SSB)
proteins bind to each single-stranded DNA, stabilizing
them  and preventing them from reforming double-stranded DNA by complementary base pairing.The RNA primer made by DNA primase is at the  end of the new strand being synthesized on the bottom template strand. The DNA primase at the fork synthesizes another RNA primer, this one on the top template DNA strand. Each RNA primer is extended by the addition of DNA nucleotides by DNA
polymerase III. The polymerases displace bound SSB proteins as they move along the template strands. The new DNAs synthesized are complementary to the template strands.
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