Characterization of canine stem cells (MSCs).

Flow cytometry is used to determine a pattern of surface markers present on a population of MSCs. The histogram on the left illustrates negative staining for the marker CD34, while the histogram on the right illustrates positive staining for CD90 (positive cells shown in red, shifted to right).
PANEL B: The Colony Forming Unit (CFU) assay is used to compare preparations of MSCs. The same number of MSCs were plated on each dish and cultured for 21 days. Donor 1 has low CFU potential, whereas Donor 2 has high CFU potential. In general, CFU potential reflects the percentage of progenitor cells present within a preparation of MSCs.