Title: Inserting the vgp gene into a cloning vector (chapter 20) Post by: R3N-R3N on Jun 15, 2013 Part B - Inserting the vgp gene into a cloning vector
In the second step of the cloning procedure, DNA fragments produced by a restriction enzyme are inserted into a cloning vector that has been cut by the same enzyme. The vector used in this tutorial is a plasmid. To clone the vgp gene into the plasmid below, a restriction enzyme must do all of the following: cut the human DNA on both sides of the vgp gene, but not inside the gene cut the plasmid without cutting inside the ampicillin resistance gene (ampR ), shown in blue (The importance of the ampR gene will be explained in Part C.) produce sticky ends when it cuts both the human DNA and the plasmid, so that the human DNA fragments can combine with the plasmid The questions below will help determine which restriction enzyme should be used to make a plasmid that contains both an intact vgp gene and a functional ampR gene. Answer questions 1–4 by selecting from the answer choices on the left. Drag the correct answer to the right of each question. Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: zoeksyrianos on Jun 15, 2013 a) BamHI - Yes, produces sticky ends, and cuts around the gene
b) HindIII - Yes, produces sticky ends, and cuts around the gene c) EcoRI - No, produces sticky ends, but cuts IN the gene d) HaeIII - No, produces blunt ends, but does cut around the gene Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: ochorios on Jun 15, 2013 Content hidden
Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: George on Jun 15, 2013 1)BamHI , Haelll and HindIIl
2) BamHI, EcoRI, and HaeIII, 3) BamHI, EcoRI, and HindIII, 4) BamHI only. Just finish my home work You can trust this answer :-* Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: R3N-R3N on Jun 15, 2013 Thank you so much ochorios and our guest George both of you were correct.. both of you are life savers..Bio has been killing me :(
Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: ochorios on Jun 15, 2013 noooo problem ^-^
Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: thegutte11 on Nov 9, 2013 Excellent, thank you!!!
Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: datly on Nov 17, 2013 Thanks for the help guys and the explanation really clarified everything
Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: superclumsy on Nov 17, 2013 Thank you ♥
Title: Re: Inserting the vgp gene into a cloning vector (chapter 20) Post by: shayla johnson on Apr 27, 2020 GREAT THANK YOU
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