Upper-Year Courses

What does it mean by induced/uninduced cultures?

What does it mean by induced/uninduced cultures?

When growing recombinant proteins I used a uninduced control culture and a auto induction medium in my production culture.

what does it mean by induced/uninduced

GCUQMU   1304   N/A

GCUQMU

Multiplex pcr for methicillin resistant s.aureus

Multiplex pcr for methicillin resistant s.aureus

i am doing  multiplex pcr for MRSA strains in three groups I wanted to know the positive control or a standard that can be run in every set to assure that the pcr reaction has worked to optimal standardization.

pathak   1108   1 Reply

habiba

Virus sample transport and preservation

Virus sample transport and preservation

can someone suggest how to transport virus seed also method of preservationand maintainance of that seed

pathak   1747   5 Replies

psyche360

Shewanella Oneidensis Sonication

Shewanella Oneidensis Sonication

Hello,

I plan to use sonication to break up my Shewanella and E.Coli microbes into their various components. I was wondering if anybody knows of a website where I can see specific instructions on how to do this. The instructions should inc

KSci   695   1 Reply

duddy

Biotechnology - chapter 20

Biotechnology - chapter 20

whats the differences between bacteria express system and eukaryotic cloning and express system?

abertista   1924   1 Reply

habiba

How to tell if a person is AA, Aa, or aa using gel electrophoresis results?

How to tell if a person is AA, Aa, or aa using gel electrophoresis results?

What in particular do I have to look for to classify a person into a particular genotype? I have attached a picture of the results to make my question a little bit easier to understand.

tingc95   1732   1 Reply

Microbiojuan

Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.

Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.

In class I did a sds-page electrophoresis of e.coli expressed in four different sugars; Frucose, Arabinose, Glucose and Galactose, all in a concentration of 30 µl. When looking at my sds-page result, the four different samples seem to express the sam

Woahscience   2615   N/A

Woahscience

Would you please write a discussion on this carbon NMR and identify the signals.

Would you please write a discussion on this carbon NMR and identify the signals.

I know the structure of product is D- Glucose and i need to explain every signal and peak. Please write a discussion about this Spectrum that related to D- glucose.
This specturm belong to my Lab report,please explain everything on detail that h

Amin.noorian   1691   3 Replies

duddy

please write a full discussion in this H Nmr and explain each signal

please write a full discussion in this H Nmr and explain each signal

I need help on H NMR ,i have to writea discussion on this specturm,I know the structure of product is D- glucose, but i could not write a discussion on it. Please explain everythingabout theses spectrum and how each signal related to the D- Glucose.

Amin.noorian   1231   N/A

Amin.noorian

How to calculate the stock material in PCR?

How to calculate the stock material in PCR?

Hi! I have a problem with filling this PCR table. Can anyone help me, please?

notimp   3435   N/A

notimp

Expression data - how to deal with it?

Expression data - how to deal with it?

Hello dear forum
I am working currently with the expression analysis of various genes by Affymetrix microarray chips. Here I come to terms with which I unfortunately can not do much.

In one illustration the absolute expression is spec

Jaccy   4552   N/A

Jaccy

Polymerase Chain Reaction

Polymerase Chain Reaction

I conducted a a polymerase chain reaction last week in my biomedical class. We were amplifying TPA-25 ALU.

A question which I don't know how to answer is what amplified fragments of approximately 400 and 2000 bp conclude?

dab353   2296   2 Replies

dab353

Restriction Digestion and Agarose Gel Electrophoresis of Plasmid DNA

Restriction Digestion and Agarose Gel Electrophoresis of Plasmid DNA

I completed a lab in which an isolated plasmid DNA was used for the restriction analysis using two different restriction endonucleases. One enzyme was BamHI and the other EcoRI. After performing a restriction digestion in combination with agarose gel

dab353   2849   4 Replies

bio_man

Why do you pour the ethanol SLOWLY in DNA extraction experiment?

Why do you pour the ethanol SLOWLY in DNA extraction experiment?

I am talking about the standard DNA isolation experiment from kiwis and onions.Protocol goes something like this (general and conceptual version follows)

1) Chop the kiwi/onion up finely and add it to a solution of NaCl and detergent

Gardenia   2141   1 Reply

bio_man

Design an experiment outlining your techniques that will analyze the temporal expression pattern of

Design an experiment outlining your techniques that will analyze the temporal expression pattern of

Design an experiment outlining your techniques that will analyze the temporal expression pattern of a gene (gene X - mRNA expression) and its protein localization during gastrulation in three different organisms.
Please state the organisms you a

gkor29   3129   N/A

gkor29

How to use STEREOLOGY (optical fractionator) in image stacks (confocal microsc.)

How to use STEREOLOGY (optical fractionator) in image stacks (confocal microsc.)

Hi! I'm using stack images after confocal microscopy to measure 2 types of cells in mouse brain after status epilepticus. PV cells (red channel) and VVA cells (green channel). I also want to measure their co-localization. Which is the best way t

anikapl   1413   1 Reply

kconnors

RNA isolation from brain sections

RNA isolation from brain sections

Hi,

I'm looking to isolate RNA from brain sections under two conditions. First are punches from fresh vibratome slices. I've been told that the time it takes to cut the sections and then punch them is too long for good RNA isolat

biomart   1313   1 Reply

rsb

how to dissolve uric acid?

how to dissolve uric acid?

Hi,
I want to dissolve uric acid ?. I need to dissolve uric acid for isolating uricase producing bacteria or uric acid utilising bacteria. when i prepare nutrient media with uric acid, it's getting sediment-ed down in conical flask...how to

barathvaj   3458   1 Reply

bio_man

A&P 2 Lab Effect of Exercise on Arterial Pressure and Vascular Resistance

A&P 2 Lab Effect of Exercise on Arterial Pressure and Vascular Resistance

Laboratory Report
Activity:
Effect of Exercise on Arterial Pressure and Vascular Resistance

Predictions
Exercise will cause MAP to
increase
Exercise will cause systemic vascular resistance to
decrease
Exe

phxguy   16734   4 Replies

Takota

ACCT410 Questions

ACCT410 Questions

E2-3   (Fund and Nonfund Accounts Identification) Indicate the fund or nonfund accounts that should be used to account for each of the following:
1.   Tax revenues restricted for road maintenance. Special Revenue Funds
2.   Resources restricted f

amashlee88   15040   2 Replies

Janie73004

Frog cardiovascular Exercise 6 in PhysioEx 8

Frog cardiovascular Exercise 6 in PhysioEx 8

I need help with frog cardiovascular exercise 6 in physio ex 8.0 =( help please!!!

afields5   3117   4 Replies

bio_man

Why did the PCR sample not show in this Electrophoresis?

Why did the PCR sample not show in this Electrophoresis?

Typical Bio lab... We transformed e.coli to having the pGreen gene and glowing in the dark. You'll see on the photo the first column we used a MWS and the second column was the pGreen +ampicillin mixture and we were able to isolate the gene to s

yetiintheback   951   1 Reply

rsb

Titrations and Natural Acid/Base Indicators

Titrations and Natural Acid/Base Indicators

Lesson 6 Lab: Titrations and Natural Acid/Base Indicators


Begin by viewing the following Thinkwell videos

15.1.7  CIA Demonstration: Titrations
11.2.2  CIA Demonstration: Natural Acid-Base Indicators

shirokyv   5551   8 Replies

padre

TTEST calculations help!

TTEST calculations help!

Hi there, I had generated a knockdown cell pool using shRNA against my target gene and control. i then subject these cells to the same conditions (DNA damage, etc).

I run experiments in triplicate usually, and then generate them independen

mm123an   2366   N/A

mm123an

Formulation of a questionnaire based on Birth Order and Coping with Stress

Formulation of a questionnaire based on Birth Order and Coping with Stress

Pleasant Good Day to All,
I am doing my Thesis on the topic Birth Order and Coping. I am in the process of trying to put a questionnaire together that helps match my independent and dependant variables: Ordinal Position, Archetypical Profile, D

Rhyanbeau   823   N/A

Rhyanbeau

Ok I need some in sight

Ok I need some in sight

We are doing Western blots in lab and I have a protein with MW of 80,000 but I am having a hell of a time finding out what it is.  Is there a site that could help me?

gardenlife1   1096   3 Replies

gardenlife1

Enzyme for the synthesis of L-ascorbic acid

Enzyme for the synthesis of L-ascorbic acid

I am doing some university research which requires an enzyme that can produce L-ascorbic acid as the end product. The problem is that my knowledge of biology is limited and I have no idea if there is one available that does this. I have searched the

Goosebay   1167   1 Reply

bio_man

Identification of Serum Glcoprotein by Polyacrylamide Gel Electrophoresis

Identification of Serum Glcoprotein by Polyacrylamide Gel Electrophoresis

Can anyone please help me with these lab questions?
These are questions from the lab called: Identification of Serum Glcoprotein by Polyacrylamide Gel Electrophoresis and Albumin Western Blotting.

1. Describe the results of your Coom

12

queenhoa   4035   15 Replies

sinemsi

What are CFU assays?

What are CFU assays?

What are CFU assays and what are they used for?

Thank you =)

ehd123   1162   6 Replies

ehd123

Preparig Histological Sections

Preparig Histological Sections

When we are preparing histological sections,
Are the Zenker & Bouin fixatives?
and if they are fixatives , are they complex fixatives ?

Aya-AlShreief   896   3 Replies

bio_man

How can I prepare a permanent slide of metaphase chromosomes from grasshopper?

How can I prepare a permanent slide of metaphase chromosomes from grasshopper?

Hello,

I have been attempting to prepare permanent mitosis and meiosis slides for both plant and animal models. I have used onion root tip, broad bean, drosophila and cricket. However I have had very little success with animal chromosomes.

Miqi   1068   3 Replies

padre

what is the standard protocol to carry out alpha amylase inhibition assay?

what is the standard protocol to carry out alpha amylase inhibition assay?

Hi. I am currently working on alpha amylase inhibition assay using DNS reagent.
As the first step I am preparing the DNS reagent and testing it with glucose to ensure that the DNS reagent is prepared in a correct way.
After boiling the mixt

SMG   1067   1 Reply

bio_man

DNA fiber analysis protocol for s. pombe?

DNA fiber analysis protocol for s. pombe?

Hi,
Does anyone have experience on DNA fiber analysis (combing) for S. pombe organism? I need a thorough protocol for that if any one can spare one or leave a link to.

Thanks,
Parham

parham   756   2 Replies

duddy

Problem with Fibroblasts cell culture from a skin biopsy

Problem with Fibroblasts cell culture from a skin biopsy

Hi everyone!

Hope that someone can help me with this issue:
I have been trying to obtain fibroblast cells from a 3,5 mm skin biopsy from belly skin and from behind the ear with an enzyme digestion protocol.
Even though we can see

CelesteAvalos   835   2 Replies

savio

Is it possible to extract hormones from plants? If so, what equipment is needed?

Is it possible to extract hormones from plants? If so, what equipment is needed?

Hey, I am new to this forum, so I don't know if anyone can answer this question, but is it possible to do what I described above? I was just wondering because I had this experiment in mind and would like to implement it if it is feasible.

Edwin96   944   4 Replies

Edwin96

PCR REACTION

PCR REACTION

I accidentally used 10 ul of master mix/DNA solution instead of 20 ul. The PCR reaction is set to calibrate temperatures for 20 ul of solution. Will my DNA samples be denatured to a point where they can't replicate?

JFKgravy   847   1 Reply

padre

Gas Chromatography Lab Question

Gas Chromatography Lab Question

Hello,

I am currently trying to analyze my gas chromatography (GC) results. In the lab, I am trying to figure out what fatty acids are found in olive oil. In the lab manual it says "you should compare the percentages (percent composit

krustythe   859   2 Replies

doubleu

Primers for Fungus, sequencing and identification

Primers for Fungus, sequencing and identification

I am trying to isolate fungi grown on an insect and identify them. Usually, for bacteria that we used in the class, I isolated the strain, extracted DNA, ran and amplified PCR and sent it to nearby university to sequence it. I know the general proces

Zone88   725   2 Replies

habiba

what is the standard curve finding concentration and absorbance

what is the standard curve finding concentration and absorbance

What is the standard curve finding concentration and absorbency

scwong   686   1 Reply

duddy

apoptotic cell identification

apoptotic cell identification

Hello everyone,

What assays can we use to detect apoptosis in cells?
I know of the annexin 5 caspase assay. But what other methods are available?
Does trypan blue help identify dead cells? What about flow cytometry?

ehd123   778   3 Replies

bio_man