Gene Therapy Department Question

You could proceed in either of two ways.  1) Simplest: isolate hematopoietic stem cells using a fluorescence-activated cell sorter, culture them, knock in a functional rag2 gene using an appropriate construct engineered for drug selection and counter selection, and reinject the transformed cells into her bone marrow.  2) More complicated: if she does not have culturable HSCs, you could culture somatic cells from one of her tissues, remove nuclei with a micropipette and inject them into previously enucleated human oocytes, culture the resulting embryos to the blastocyst stage, culture ES cells from these blastocysts, knock in a functional rag2 gene as in (1), treat the transformed ES cells with appropriate growth factors to produce HSCs, and inject these back into her bone marrow.