Splicing Question

juno1973

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9 years ago

Splicing Question

A patient with skin lesions and extreme sensitivity to UV visits a doctor. A skin biopsy is performed and genomic DNA is isolated and sequenced for Xeroderma Pigmentousum gene mutations. The results indicate a mutation in XPA gene, which consists of 7 exons and 6 introns. This patient has a mutation that occurs at the 5' splice site of exon 2.
1) Draw the pre-RNA including introns and eons of XPA and indicate where the mutation is located.
2) Draw out what the predicted mRNA transcript of this patient would look like.

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