1.You work in a laboratory that regularly analyzes patient specimens for the presence of Clostridium difficile.
a. Knowing that many specimens test positive, explain which type of antimicrobial chemical agent you should choose to effectively clean your laboratory each day.
b. Provide an explanation in support of or refuting the flowing statement: “The goal of disinfection is the destruction of bacterial endospores.” Briefly explain how the type of microorganisms present will influence the effectiveness of an antimicrobial agent.
2.a. Discuss the factors that influence an antimicrobial agent’s ability to completely remove microbial contaminants from dentures used on a daily basis.
b. Improper cleaning of endoscopes has led to the transmission of hepatitis C infection among patients given colonoscopies at the same facility. Develop one hypothesis to explain how the virus may have persisted and remained infectious.
3.a. Precisely what is microbial death?
b. Why does a population of microbes not die instantaneously when exposed to an antimicrobial agent?
4. Compare and contrast the terms bactericidal and bacteriostatic in relation to microbial control agents. Develop a simple experimental method that could be used to test whether a control agent exhibits bactericidal or bacteriostatic effects.
5.a. Provide a valid reason for whether or not heat is a selective microbial control agent.
b. Explain the concepts of TDT and TDP, and discuss one example of how these measurements can be modified to produce food for human consumption that is free of microbial contaminants yet still tasty, using examples. What are the minimum TDTs for vegetative cells and endospores?
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