You need to login first

Login information

Haven't yet registered?

×
* * * *

Donate

Your donation is safe and helps support the issues and causes you care about most.
Pages: 1  Down
  New Topic  |  Print  
physioex 9 exercise 12
Read 2492 times | 2 Replies | Average Rating: Not Rated Yet
jerikamiller
Jellyfish
*
Posts: 14
Points: 45
Rep:  +0   -0

Joined: Jan, 2012


Seeking a more comfortable homework help environment? Homework Clinic
I just realized my physioex 9.0 exercise 12 sheet is due today and I dont think I have time to finish it because I have a million other things I need to do. If anybody has any of the answers and could help I would greatly greatly appreciate it! I really need to get it done. Im usually very good at staying on top of my work but this week has been hectic and this assignment completely slipped my mind! Here are the questions..

Activity 1
1. -
2. Explain where the epitope (antigenic determinant) is located.
3. Describe how a positive result is detected in this serological test.
4. How would the results be affected if a negative control gave a positive result?

Activity 2
1. Describe how you were able to determine what antigen is in the unknown well.
2. Why does the precipitan line form?
3. Did you think human serum albumin and bovine serum albumin would have epitopes in common?

Activity 3
1. Describe how the direct and indirect ELISA are different.
2. Discuss why a patient might test indeterminate.
3. How would your results have been affected if your negative control had given an inderterminate result?
4. Briefly describe the basic structure of antibodies.

Activity 4
1. Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV.
2. -
3. Briefly describe how the nitrocellulose strips were prepared before the patient samples were added to them.
4. Describe the importance of the washing steps in the procedure.

LoggedReport this PostReport Abuse

Reply# 1
Posts: 129
Points: 1317
Rep:  +12   -1

physioex 9 exercise 12
Mar 15, 2012

2. Discuss why a patient might test indeterminate.

The patient might test indeterminate if they have not yet seroconverted or if they have not yet produced enough antibodies to yield a positive result.

3. How would your results have been affected if your negative control had given an inderterminate result?

If the negative control is positive, this invalidates of the results. It could be due to not enough washing in the washing steps to remove nonspecific binding.



Report this PostReport Abuse
Reply# 2
Posts: 14
Points: 45
Rep:  +0   -0

physioex 9 exercise 12
Mar 15, 2012

Thank you!



Report this PostReport Abuse
Pages: 1    Up
  New Topic  |  Print  
 
Related Topics
Replies Author Last post
5
Replies
Physioex 8.0 Exercise 4 in Anatomy and Physiology
Started by magee_lauren | Views: 7273
Last post Last post by duddy
Jan 22, 2011
1
Reply
PhysioEx 8.0 Exercise 4 in Upper-Year Courses
Started by ermosley1 | Views: 3910
Last post Last post by duddy
Feb 6, 2011
73
Replies
PhysioEX 8 Exercise 7 ... in Anatomy and Physiology
Started by mb2005 | Views: 42113
Last post Last post by brookep
Sep 10, 2012
10
Replies
physioex 9.0 Exercise 7 Respiratory Systems Mechanics & Exercise 9 Renal System in Anatomy and Physiology
Started by bionicman | Views: 28300
Last post Last post by raininginny
Feb 16, 2012
Note: This topic is currently locked from adding new posts. Only administrators and moderators can reply. If you'd like to contribute to this topic, start a new thread and make reference to this one. Otherwise, contact a moderator for more options.