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Home Q & A Board Laboratory Help Introductory Courses
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kondzia kondzia
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9 years ago
Why is it necessary to substract this absorbance from all other results?
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#1
Valued Member
On Hiatus
9 years ago
I am not sure but try if this helps  Slight Smile

http://www.piercenet.com/method/protein-assay-data-analysis
rsb
wrote...
#2
9 years ago
Quote
Why does the blank have an absorbency reading above zero?

It should be defaulted to zero.

You subtract it to get the difference.
wrote...
#3
Educator
9 years ago
If it has a reading of zero, that means there is full transmittance, which is what we want, a transparent blank that we can compare our results to.
wrote...
#4
Staff Member
9 years ago
Because it absorbs! Slight Smile That's why we need to have a blank before we take the absorbance reading of a sample. We don't want to know the absorbance of the sample, not the blank. And we can be sure that we are measuring the absorbance of only the sample by subtracting out the blank's absorbance from the absorbance of your samples. Your spectrophotometer or whatever you are using may do the subtractions automatically for you.
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