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in protein chemistry,what would the function of the following reagents be?

Tebatso398

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Posts: 1

Rep:

9 years ago

in protein chemistry,what would the function of the following reagents be?

ninhydrin urea trypsin sodium dodecyl sulphate.B-mercaptoethanol

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psyche360

wrote...

#1

9 years ago

Quote from: Tebatso398 (9 years ago)

ninhydrin

Ninhydrin is also used in amino acid analysis of proteins. Most of the amino acids, except proline, are hydrolyzed and react with ninhydrin. Also, certain amino acid chains are degraded. Therefore, separate analysis is required for identifying such amino acids that either react differently or do not react at all with ninhydrin. The rest of the amino acids are then quantified colorimetrically after separation by chromatography.

Quote from: Tebatso398 (9 years ago)

urea

I believe it denatures the protein, but I'm not entirely sure.

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