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Need a little help on PhysioEx 9.0, Exercises 8, Chemical and physical processes of digestion

The following questions refer to Activity 2: Exploring Amylase Substrate Specificity.

1. Describe why the results in tube 1 and tube 2 are the same.
2. Describe the result in tube 3. How well did the results compare with your prediction?
3. Describe the usual substrate for peptidase.
4. Explain how bacteria can aid in digestion.

The following questions refer to Activity 3: Assessing Pepsin Digestion of Protein.

1. Describe the effect that boiling had on pepsin and how you could tell that it had that effect.
2. Was your prediction correct aboutthe optimal pH for pepsin activity? Discuss the physiological correlation behind your results.
3. What do you think would happen if you reduced the incubation time to 30 minutes for tube 5?


The following questions refer to Activity 4: Assessing Lipase Digestion of Fat.
1. Describe the activity of lipase with and without the addition of bile salts. Refer to Chart 4 for pH values.
2. Is the activity of bile a chemical or a physical process? Explain.
3. What pH resulted in the maximum pancreatic lipase activity? How does this optimal pH correlate to the enzyme’s location in the body?
4. Explain whether or not we can determine fat hydrolysis in tube 5. Why or why not?

Any help would be greatly appreciated
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1. Describe why the results in tube 1 and tube 2 are the same.
2. Describe the result in tube 3. How well did the results compare with your prediction?
3. Describe the usual substrate for peptidase.
4. Explain how bacteria can aid in digestion.

1. No, amylase doesn’t use cellulose as a substrate. Starch is the substrate for amylase.
2. The bacteria were able to digest the cellulose because they produce the enzyme, cellulase.
3. The peptidase had no effect on the starch because the substrate for peptidase is peptides and proteins.
4. The smallest subunit that starch can be broken down into is glucose.

1. Describe the effect that boiling had on pepsin and how you could tell that it had that effect.
2. Was your prediction correct aboutthe optimal pH for pepsin activity? Discuss the physiological correlation behind your results.
3. What do you think would happen if you reduced the incubation time to 30 minutes for tube 5?

1. The optimum pH matches the pH secreted by gastric glands. Gastric juice is also about pH 2.
2. Since the pH of the mouth is closer to neutrality, you would expect pepsin to be slightly active but not as
active as it is in the stomach at pH 2.
3. The subunit products of digestion are peptides and amino acids.
4. The control tube #4 is present to make certain that the BAPNA is not breaking down due to the low pH.

1. Describe the activity of lipase with and without the addition of bile salts. Refer to Chart 4 for pH values.
2. Is the activity of bile a chemical or a physical process? Explain.
3. What pH resulted in the maximum pancreatic lipase activity? How does this optimal pH correlate to the enzyme's location in the body?
4. Explain whether or not we can determine fat hydrolysis in tube 5. Why or why not?

1. Lipase activity is measured by a decrease in pH through the release of fatty acids.
2. The pH in tube #5 is pH 2.0. The pH is too low to be able to see if any fatty acids have been released.
3. Pancreatic lipase would be active in the mouth since the pH of the mouth is close to 7.0 and the enzyme is
most active at this pH.
4. Fat globules are separated into droplets by bile salts through an emulsification process which is physical
not chemical.
- Master of Science in Biology
- Bachelor of Science (Biology)
- Bachelor of Education
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2 years ago
Are you sure these are for answers for PhysioEx 9.0 Huh?  The questions are different then 8.0.

Activity 2: Exploring Amylase Substrate Specificity.
1. Describe why the results in tube 1 and tube 2 are the same. Is not a yes or no question.

you have 4 answers for activity 3 and there is only 3 questions PhysioEx 9.0  Confused

I appreciate the quick response but I'm a little skeptical of the answers, and I will continue to do the exercises. Checking back to confirm what answers I come up with the ones you have provided.  Cool

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2 years ago
Duddy is correct, my version of PhysioEx 9 is the same.
Biology!
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What are you talking about...look at what I posted, ok let's break this down.

PhysioEx 9.0, Exercises 8, Chemical and physical processes of digestion

The following questions refer to Activity 2: Exploring Amylase Substrate Specificity.

1. Describe why the results in tube 1 and tube 2 are the same. This is not a yes or no question!

1. No, amylase doesn't use cellulose as a substrate. Starch is the substrate for amylase. Huh?

2. Describe the result in tube 3. How well did the results compare with your prediction? Tube 3 is Amylase ans Cellulose....Not Bacteria

2. The bacteria were able to digest the cellulose because they produce the enzyme, cellulase.Huh?

3. Describe the usual substrate for peptidase. What is the USUAL   substrate for peptides?

3. The peptidase had no effect on the starch because the substrate for peptidase is peptides and proteins.

4. Explain how bacteria can aid in digestion.  EXPLAIN....so bacteria....is the smallest subunit aiding in digestion???

4. The smallest subunit that starch can be broken down into is glucose.

The following questions refer to Activity 3: Assessing Pepsin Digestion of Protein.

1. Describe the effect that boiling had on pepsin and how you could tell that it had that effect. What glands, Gastric juice?Huh??

1. The optimum pH matches the pH secreted by gastric glands. Gastric juice is also about pH 2.

2. Was your prediction correct about the optimal pH for pepsin activity? Discuss the physiological correlation behind your results. This looks good!!!

2. Since the pH of the mouth is closer to neutrality, you would expect pepsin to be slightly active but not as
active as it is in the stomach at pH 2. looks goods


3. What do you think would happen if you reduced the incubation time to 30 minutes for tube 5? There not asking about subunit products.

3. The subunit products of digestion are peptides and amino acids.Huh???

There is no question 4, in PhysioEx 9.0, Exercise 8 this sends up a red flag.

4. The smallest subunit that starch can be broken down into is glucose.Huh??? again no question 4...


Do you see what I mean?

The Review sheet for Exercise 8 is on page 127 and 128,   

ISBN-13: 978-0-321-81557-6   OR    ISBN-10 0-321-81557-2

just check the book out and see if your using the right one.....please.
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There is no question 4, in PhysioEx 9.0, Exercise 8 this sends up a red flag.

Homer: I noticed that too, but Duddy posted the answers that correspond to the activities you needed help on. Whether or not they are in the correct order, that's up for debate.
Biology!
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The following questions refer to Activity 3: Assessing Pepsin Digestion of Protein.

1. Describe the effect that boiling had on pepsin and how you could tell that it had that effect.
2. Was your prediction correct aboutthe optimal pH for pepsin activity? Discuss the physiological correlation behind your results.
3. What do you think would happen if you reduced the incubation time to 30 minutes for tube 5?


The following questions refer to Activity 4: Assessing Lipase Digestion of Fat.
1. Describe the activity of lipase with and without the addition of bile salts. Refer to Chart 4 for pH values.
2. Is the activity of bile a chemical or a physical process? Explain.
3. What pH resulted in the maximum pancreatic lipase activity? How does this optimal pH correlate to the enzyme's location in the body?
4. Explain whether or not we can determine fat hydrolysis in tube 5. Why or why not?

Sorry about the confusion, but here's the part...

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- Master of Science in Biology
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2 years ago
I think I know, were I went wrong. I need the Review Sheet questions. Maybe this will help?
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Quote
List the substrate and the subunit product of amylase.

The substrate of amylase is animal starch and the product is maltose and glucose.

Quote
What effect did boiling and freezing have on enzyme activity? Why? How well did the results compare with your prediction?

The boiling denatured the enzyme and inactivated it as predicted. The freezing has no effect on the enzyme.

You mean these? Thinking
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2 years ago
duddy, I think our books are different. My activity questions are not the same as yours.
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2 years ago
Were all the exercise 8 review questions posted?
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2 years ago
this was all very much confusing  Huh?
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thanks for the help guys! It helped me understand a little more  Wink
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The answers were out of order but I figured it out. Thanks!
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