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06Ralph 06Ralph
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11 years ago
After I plotted the graph,the graph is a curve on the 1st quadrant which cuts neither the x or y axis.In this case,what are the steps and formulas to calculate the initial rate of the enzyme reaction?Thanks.
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11 years ago
Measure the gradient of the curve at different times.
Then plot a second graph of time (x-axis) vs the calculated rates (y-axis) and you should get a straight line. Extrapolate the line to the y-axis where x = 0. This is your initial rate.
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