two-dimensional gel electrophoresis

minjai0322

wrote...

13 years ago

There are two proteins; one has a molecular weight of 10,000 Dalton and a pI of 7.1. The other has MW of 40,000 Da and pI of 4.9. They are separated by two-dimensional gel electrophoresis. The first dimension is PAGE. The second dimension is on a pH gradient gel for isoelectric focusing. Sketch their likely relative position at the end of the gel electrophoresis.

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sarah!

wrote...

13 years ago

Here is my guess Sad Dummy

would love to know the way you did it minjai0322.

Please reply back, my version is attached.

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minjai0322 Author

wrote...

13 years ago

Quote from: sarah (13 years ago)

Here is my guess Sad Dummy

would love to know the way you did it minjai0322.

Please reply back, my version is attached.

Could you tell me the reason why the one that has heavier molecular weight
is on the above? Shouldn't it be below because it's heavier?

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bio_man

wrote...

Educator

13 years ago

Thanks for your contribution Sarah!

The heavier the molecule, the shorter the distance it will travel. Think of something heavy running really quickly - won't go far. Whereas the smaller molecule will run farther and quicker.

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minjai0322 Author

wrote...

13 years ago

Quote from: bio_man (13 years ago)

Yeah! I got your point that the smaller molecule would travel further.
But, what I saying is that the position of both molecules, which is above and which is below.
Anyway, I appreaciate for your help, sarah and bioman!
Thanks a million~!

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bio_man

wrote...

Educator

13 years ago

Oh that's assuming the proteins were injected into the same well of the SDS PAGE Slight Smile

.

You're welcome!

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