Need water test for Naegleri, Ecoke. Where to buy??

Direct visualization is probably your best bet.

Direct visualization: The motile amoeba can often be seen moving under a microscope when looking at a fresh sample of cerebral spinal fluid (CSF) from an infected individual. The amoeba can also be stained with a variety of stains, such as Giemsa-Wright or a modified trichrome stain for identification.

Immunohistochemistry (IHC): An antibody specific to Naegleria fowleri can be used in conjunction with another antibody that deposits a chemical or glows under specific types of light (indirect fluorescent antibody [IFA]) to directly stain the amoeba in tissue.

Polymerase Chain Reaction (PCR): Specific molecular tools can amplify DNA from the amoeba in CSF or tissue to specifically identify its presence. Looking at strains or subtypes of Naegleria fowleri can be done, but little is known about the natural populations in the environment which makes it difficult to interpret what the findings mean.

Ameba culture: The sample is added to a growth plate covered in bacteria that serves as a food source for Naegleria fowleri. Incubating at a higher temperature selects for Naegleria fowleri growth, which can be visualized as tracks made by the ameba as it moves across the plate eating the bacteria. Growing Naegleria fowleri in mammalian cell culture and looking for toxic cell effects is also possible.

Environmental Detection: Water samples can be collected, concentrated and put into culture to grow and select for Naegleria fowleri. Samples can be tested using the serologic or molecular methods described above.