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biology1012 biology1012
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7 years ago
ChIP-seq analysis using an antibody against a
bromodomain protein on chromatin isolated from kidney glomerulus cells provides the following graph. The list on the left  provides names of the different genes being investigated. The green bars represent to extent to which the DNA sequences corresponding to gene promoters are recovered in the co-immunoprecipitation assay. Genes Aqp1 to Scnn1a represent set A whereas the the last 3 genes represent set B.

Are set A and set B genes more likely to be transcribed or repressed in kidney glomerulus cells? Explain your answer. Include:
a)   what a a bromodomain is,
b)   in which class of proteins it is found,
c)   which histone modification is implicated and
d)   what is the predicted effect on transcription.


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