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silasIII silasIII
wrote...
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7 years ago
Is there any handbook that I can find such data for example for endothelial cells of human or mouse or bovine?
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3 Replies
Replies
wrote...
Educator
7 years ago
I believe it's almost impossible, but modern day science has changed that:

If you had to estimate the mass of the average human cell, you could cheat and look it up on Wikipedia, or do a back-of-the-envelope calculation with a few assumptions about the mass and volume of a person (a cylinder) and the size of cells (spheres). But researchers need more precise measures of a cell’s mass so they can detect small changes as it grows or responds to its environment. In Physical Review Letters, Kevin Phillips and colleagues at the Oregon Health & Science University in Portland report a simple way to measure the mass of individual red blood cells with a commercial microscope.

To do this, Phillips et al. have developed a technique called tomographic bright-field imaging, which reconstructs the 3D optical index of refraction of a microscopic object, like a cell or plastic bead, and then converts this information into the object’s mass. The team places a narrow aperture in front of a microscope light source to make a unidirectional plane wave and passes this light through a glass slide containing red blood cells. A CCD camera on the opposite side of the slide maps out the light intensity. Phillips et al. feed a series of these intensity maps, each corresponding to the slide at different distances from the focal point of the light source, into a numerical model that reconstructs the 3D index of refraction of the cells. They then use tabulated values to relate the index to the mass density of the red blood cells (and some other tricks to find their volumes), arriving at a mass of roughly 27 picograms (1 picogram =10−12=10-12 gram) per cell.

Hope you find this read interesting... Smiling Face with Open Mouth
Source  https://physics.aps.org/synopsis-for/10.1103/PhysRevLett.109.118105
Answer accepted by topic starter
bio_manbio_man
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7 years ago
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This verified answer contains over 250 words.
Source  http://www.ncbi.nlm.nih.gov/books/NBK21473/
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silasIII Author
wrote...
7 years ago
Macromolecules, though, are the most interesting and characteristic molecules of living systems; in a true sense the evolution of life as we know it is the evolution of macromolecular structures. Proteins, the workhorses of the cell, are the most abundant and functionally versatile of the cellular macromolecules. To appreciate the abundance of protein within a cell, we can estimate the number of protein molecules in a typical eukaryotic cell, such as a hepatocyte in the liver. This cell, roughly a cube 15 μm (0.0015 cm) on a side, has a volume of 3.4 × 10−9 cm3 (or milliliters). Assuming a cell density of 1.03 g/ml, the cell would weigh 3.5 × 10−9 g. Since protein accounts for approximately 20 percent of a cell’s weight, the total weight of cellular protein is 7 × 10−10 g. The average yeast protein has a molecular weight of 52,700 (g/mol), as noted in Chapter 3. Assuming this value is typical of eukaryotic proteins, we can calculate the total number of protein molecules per liver cell as about 7.9 × 109 from the total protein weight and the number of molecules per mole, which is a constant (Avogadro’s number). To carry this calculation one step further, consider that a liver cell contains about 10,000 different proteins; thus, a cell contains close to a million molecules of each protein on average. In actuality, however, the abundance of different proteins varies widely, from the quite rare cell-surface protein that binds the hormone insulin (20,000 molecules) to the abundant structural protein actin (5 × 108 molecules).

Thank you for the response but In your first response the answer for mass is 27pg but in the second one it is 3.5*10^-9g. These numbers are not compatible and way different from each other.
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