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Home Q & A Board Laboratory Help Introductory Courses
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smooth15 smooth15
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11 years ago
I'm trying to make bacterial growth curve. So I was told to do serial dilutions first to determine how much to put in the cuvette for OD, but how do I calculate that number? I'm so confused. Is there a fixed cell number for OD?
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mickey27121mickey27121
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11 years ago
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11 years ago
as above but also the spectrophometer is really only accurate between 0.1 and 1 so if you measure a serial dilution you'll be sure that at some point your absorption will be between these values.

but mainly as above
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