Dideoxynucleotide DNA sequencing (Mastering Genetics)

the DNA sequence from above pattern interpreted as follows -

considee the band which is lowest from top among all the bands, that will be the 1st nucleotide at 5' end of DNA sequence . Here its the band in A lane. Thus A is the 1st nucleotide of DNA sequence (5'-3')

then consider the 2nd most lowest band among all the bands, that will be the 2nd nucleotide of DNA sequence. Here T is the 2nd most lowest band and hence is the 2nd nucleotide.
now continue the pattern of reading the pattern and find the DNA sequence
Thus based on above method, the sequenced strand is as follows -

---> 5'- ATGGACCAGTTG -3'

# So the complementary template strand of this sequenced DNA is as follows -
----> 5' - CAACTGGTCCAT - 3'

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