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hutld hutld
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7 years ago
assuming that all other variables remain constant explain why light of shorter wavelengths will produce a clearer image that light of longer wavelengths?
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Educator
7 years ago
Hi

Limit of resolution (D) = wavelength / (NA condenser + NA objective)

A small wavelength will allow for a smaller D value. Limit of Resolution is defined as the distance apart two objects must be for the microscope to distinguish them as separate objects. A small D value means better resolution, thus better clarity.
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