Vector Cloning

allan2009

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13 years ago

Vector Cloning

A vector was prepared by a double restriction digest (EcoR1 + BamH1) to generate different ends for zero background colonies. Say you preformed a parallel experiment using the different enzyme pair Sma1 and Hae111 to prepare the vector, which was then ligated to fragments with Sma1/Hae111 ends. What result would be obtained when the transformation plates were examined? 4marks... i have know idea, any feedback would help.

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