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iksun1002 iksun1002
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Posts: 59
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10 years ago
We did this osmosis lab at school, we took decalcified eggs and placed one in water, molasses, 0.1M glucose solution, 0.5M glucose solution, and 1.0M glucose solution. The results were as expected; the molasses egg lost weight significantly, the water egg gained the most weight, and the eggs in glucose all gained weight but as the molarity increased, the gain of weight decreased. How does osmotic pressure and osmotic gradient relate to this experiment? Thanks!
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wrote...
10 years ago
Imagine two containers separated with a semipermeable membrane, permeable to water only. Left side contains a 1M NaCl soln, while the right contains a 2M NaCl soln. The water will move from the left into the right, right? Why did it do so in the first place? It was because of the OSMOTIC GRADIENT. There was a difference in NaCl concentrations, creating a gradient, and thus inducing osmosis to the right container. Osmotic pressure then, is the pressure you would have to apply to keep the water from going to the right side. In your experiment, the osmotic gradient is provided by the molasses/glucose solutions, relative to the egg. And the greater the gradient, the greater the osmotic pressure (the more pressure the walls of the egg have to exert to keep water from coming in/out). So the molasses/water parts would induce the greatest gradient and therefore the greatest pressure (because a lot of water left/entered).
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