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colleen colleen
wrote...
Valued Member
Posts: 17077
10 years ago
Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA. Assume also that the restriction endonuclease Cuj cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000, 1500, and 3000. Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced, what sizes of DNA are expected?
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4 Replies
Sunshine ☀ ☼
Replies
wrote...
10 years ago
500 bp, 1500 bp, and 2000 bp
Answer accepted by topic starter
nite_n_daynite_n_day
wrote...
Top Poster
Posts: 4086
10 years ago
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colleen Author
wrote...
Valued Member
10 years ago
Fantastic!!
Sunshine ☀ ☼
wrote...
3 years ago
thank you
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