Phyllosphere Project[4329]

Phyllosphere Project Ben, Emelia, Dylan, Vince Background Phyllosphere: total above ground portion of plants as the habitat for microorganisms “the leaf surface” (Bacterial Diversity in Tree Canopies of the Atlantic Forest) Phyllospheres vary between species Our Research Cercis canadensis Variables Independent: Area that we extracted bacteria Nutrient/Levine EMB A/Mannitol salt Dependent: amount of bacteria/type of bacteria Techniques Bacteria Extraction Avoid contact Immediately in sterile tube Electrophoresis Results Electrophoresis {5DA37D80-6434-44D0-A028-1B22A696006F}Class Group A Group B Class 1 82 85 Class 2 76 88 Class 3 84 90 First bullet point here Second bullet point here Third bullet point here PCR Results {5C22544A-7EE6-4342-B048-85BDC9FD1C3A}Lane 1 2 3 4 5 6 7 Marker DNA 2-2B 2-2 B 2-3 A 2-3 A 2-4 C 2-4 C Various Sizes Primer Dimer Primer Dimer Primer Dimer 500 B.P. 500 B.P. Primer Dimer Primer Dimer Blast BLAST Results {5C22544A-7EE6-4342-B048-85BDC9FD1C3A}BP 159 170 173 175 176 194 204 209 210 218 220 225 228 116 A C A A A A C T C T C A G 119 G T G G T G T G A G A T T Hypothesis We predicted we would find different epiphytes in differing phyloshperes Our research supported our hypothesis, as we found that epiphytes differed in different locations Concepts and results All phylospheres are not the same Many factors influence the make up of a phylosphere – location, climate etc. Some swabbing issues, not a huge sample was collected – inconsistencies Future, more in depth study could be performed to pinpoint the phylospheres of exact locations on the tree