schoool practicum explaining different diseases and how they are to experiment

2-3 sentences restating the research question (capstone presentations) or topical focus (posters). 2. 3-4 sentences reviewing the proposed methods to address the research question (capstone) or the range of philosophical or applied research approaches that are explored in the poster 3. 2-3 sentences stating the future direction of the work OR the present results/findings/conclusions. What leukemia is Current treatnment therapies Incidence of relapse Immunetherapy Leukemia is a disease that begins in the bone marrow. Leukocytes do not develop properly, leading to an overproduction of premature leukocytes. All trypically develops in utero as a result of a chromosomal translocaion, such as the bcr-abl gene fusion Round 1: Chemotherapy agents: vincristine: prevents proper cell division and repoication, leading to cell death Doxorubicin targets phases of the cell cycle to inhinit cell growth and replication Round 2: Hemtopoietic stem cell ttransplantation and more chemos Immunotherapy goal to aid the patient’s immune system in fighting off leukemic clones through blinatumomab: bispecific t cell engager. One arm designed to target CD19 on Bcell leukemia forcing apoptosis of the cancer. Dual therapy treatments study done to explore the impact of comningation therapy with mAB rituximab and HOP in the treatment of large B cell therapy Her specific needs: Combinged therapy of Binetumomab and vincristine. B-ALL cell lines from relapsed leukemia patients will be used to study effects of the combined treatment terapy more effective than murine model patient derived cell lines also can be beneficial. Viable cells will then be plated on a 96 well plate, and labeled with calcein-am before a realtime cutotoxic assay is perormed1/3 incubated in B 1/3 incubated in both and 1/3 in V Develop a monoclonal antibody similar to blinotumomab. Injecting CD10 into a mouse and remove the spleen. First Capstone Furthering treatment options for cystic fibrosis patients. Her sisters fdaughter has CF. What is cystic fibrosis:A secretory gland disorder that is geneeticall inherited Must inherit both faulty cystic fibrosis transmembrane Current treatments Bronchodilators Chest pT Controlling lung infection Lung transplant Exercise Anti inflammatories Antibiotics CFTR channel Is made withing the cell migrates toward the plasma membrane embeds itself into the membrane allowing chloride outside the cell. Epithelial sodium channels Airway surgace liquid carries the mucus to the top of the lung Specific aim 1 Identifying the amount of chloride that must be present in order to obtain adequate sodium absorption Model: mice Experimental groups: healthy, Beta-enac over expresser, Bera enac over expresser CFTR Drug denufosol sectetes chlorid independent of the CFTR. Methods chloride level: sweat test collect sweat and test it ASL height: ENAC level: sacrifice mouse, isolate and slice lung. Place in saline solution to be viewed under a stereomicroscope Clamp a single cell membrane. Specific aim 2 Using a combined drug therapy of denufosol and amiloride. Sweat test again Future studies: ORkambi Helps with understanding how long ivocafter is open to help with protein absorption. Capstone 2 Lissette Victorio Reducing treatment time and mortality rate of multidrug-resistant tuberculosis Tuberculosis is a worldwide infectious disease . Mucobacterium tuberculosis affected organs contagious disease. Two types: latent and active TB Treatment: isoniazid, rifampicin, ethambutol, pyrazinamide Causes: incorrect use of antimicrobial drugs, premature treatment interruption Culture based drugs or molecular test ~490,000 diagnosed annually expensive. (MDR) HIV A lentivirus responsible of HIV infection MDR-TB and HIV coinfection 500,000 diagnosed annually 94% mortality rate. How it starts With and HIV infected patient,,, exposed to TB,,, TB and HIV coinfection Current treatment. First stage: regular MDR-Tb treatment Second stage: treat HIV 2yr treatment time for TB causing the HIV is progressing. Aim 1: TB and HIV coinfected patients treated with Fluoroquinones will have better outcome Select 150 mice, infect mice with HIV confirmation of HIV infection. Infect mice with M. tuberculosis, CT scan. Control group 50 mice no treatment Experimental group 50 mice regular first line treatment 50 mice fluoroquinolones. Aim 2: Investigat if surgical resection after treatment with fluoroquinolones in replacement of first line drugs avoids the rate of MDR TB and HIV infections 50 mice 25 no surgical resection 25 mice surgical reection Follow up is using CT scans Goal to increase the life span of the patients Future studies: Clinical trial A more in-depth research of the mechanism of action of MDR TB and HIV coinfection Increase awareness of proper intake of these drugs Increase awareness of anti TB vaccines Capstone 3