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SlideshowReport

Attachment of gene clusters to the nuclear matrix

Description
A map of the repeating histone gene cluster in Drosophila, where each white arrow is a histone gene (arrowheads indicate direction of transcription).

If Drosophila nuclei are extracted with lithium diiodosalicylate, to remove proteins gently, and are then digested with a collection of the restriction endonucleases shown, only the 657-bp HindI-EcoRI DNA fragments are left attached to the matrix.

The interpretation is that the gene clusters exist in individual loops, the bases of which are tied to the matrix.
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