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SlideshowReport

Three-dimensional structure of citrate synthase

Description
The two forms of pig heart citrate synthase homodimer shown here were determined by crystallographic methods and support the induced fit model of enzyme catalysis.

In the absence of CoA-SH the enzyme crystallizes in an “open” form. Citrate binds at the base of large clefts in both catalytic domains of the homodimeric protein.
Binding of CoA-SH causes the enzyme to adopt a “closed” conformation, with the clefts essentially filled.
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