DNA inserted into multiple cloning site of a plasmid disrupts

In blue-white selection procedures, DNA inserted into multiple cloning site of a plasmid disrupts the lacZ gene so that bacteria containing recombinant DNA are unable to metabolize X-gal, resulting in white colonies that allow direct identification of bacterial colonies carrying cloned DNA inserts.

                      IacZ . / gene Multiple _ . ' cloning site Ampuclllm realslance (amp“) 1. Multiple cloning site of plasmid * is out with restriction enzyme. 3' 5, IacZ gene 5, / IacZ gene 2. DNA to be cloned cut with same restriction enzyme. 3.: 5' ‘ 3. DNA ligose joins together plasmid DNA and DNA to be cloned to create recombinant plasmid.     IacZ gene DNA insertion / disrupts IacZ gene / IacZ gene 4. Transform bacteria with plasmids Grow cells on media t with amplclllin and X-gal. Bacteria with nonrecombinanl plasmids are blue Bacteria with recombinant plasmids are white