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Title: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: mmurt13 on Feb 6, 2014 The diagram below shows an autoradiograph of a DNA sequencing gel.
Type the 5' to 3' sequence of the template strand (“inferred strand”) based on the pattern in this gel. Use only capital letters for the sequence. Title: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: padre on Feb 6, 2014 the DNA sequence from above pattern interpreted as follows -
--> considee the band which is lowest from top among all the bands, that will be the 1st nucleotide at 5' end of DNA sequence . Here its the band in A lane. Thus A is the 1st nucleotide of DNA sequence (5'-3') --> then consider the 2nd most lowest band among all the bands, that will be the 2nd nucleotide of DNA sequence. Here T is the 2nd most lowest band and hence is the 2nd nucleotide. --> now continue the pattern of reading the pattern and find the DNA sequence Thus based on above method, the sequenced strand is as follows - ---> 5'- ATGGACCAGTTG -3' # So the complementary template strand of this sequenced DNA is as follows - ----> 5' - CAACTGGTCCAT - 3' Title: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: alf199318 on Apr 13, 2014 Thank you!
Title: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: bellaaa on Dec 1, 2014 thankyou!
Title: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: padre on Dec 1, 2014 My pleasure guys :D
Title: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: CandyTon on Apr 24, 2015 Thanks for your answer. Learned!
Title: Re: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: Jennifer6 on May 2, 2023 Thank you.
Title: BFSF: Dideoxynucleotide DNA sequencing (Mastering Genetics) Post by: Poliann Flores on Nov 6, 2023 Thanks
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