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Title: Sort regions of the fertilized egg above based on the status of letters Post by: thamar2893 on Apr 27, 2014 Part A - Experimental technique: Tracking cytoplasmic Ca2+ distribution after a sperm enters the egg
Based on what they knew about the fertilization envelope (a protective layer that forms around the egg when a sperm fuses with it), Steinhardt, Shatten, and their colleagues hypothesized that changes in the distribution of Ca2+ ions in sea urchin eggs are involved in the formation of the fertilization envelope. In sea urchin eggs (as in most eukaryotic cells) the concentration of Ca2+ ions is much higher in the endoplasmic reticulum (ER) than in the cytoplasm. To see how cytoplasmic Ca2+ concentration changes in the egg during fertilization, the researchers injected a Ca2+-specific fluorescent dye into the cytoplasm of unfertilized eggs. After adding sperm to the eggs, they observed the eggs with a fluorescence microscope. The following images show the changes in fluorescence that occurred after a single sperm entered the egg. The fluorescence of the dye increased with increasing cytoplasmic Ca2+ concentration. The green color indicates the region of the cell with the highest fluorescence at that point in time. Title: Re: Sort regions of the fertilized egg above based on the status of letters Post by: padre on Apr 27, 2014 1) https://biology-forums.com/index.php?topic=93816.0
2) https://biology-forums.com/index.php?topic=98321.0 |