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Title: A. How can nucleosome core particles be isolated from chromatin? B. What molecular components were ... Post by: BallsDeep on Feb 3, 2018 A. How can nucleosome core particles be isolated from chromatin?
B. What molecular components were identified after this treatment was complete? C. What portion of the nucleosome was destroyed/removed during this treatment and what function does it normally serve? Title: A. How can nucleosome core particles be isolated from chromatin? B. What molecular components were ... Post by: taylorhovland on Feb 3, 2018 A. In a test tube, the nucleosome core particle can be released from chromatin by treatment with a nuclease that degrades the exposed, linker DNA, but not the DNA wrapped around the nucleosome core.
B. The core nucleosome was revealed to contain two molecules of the histones H2A, H2B, H3, and H4, as well as a 147-base-pair (bp) fragment of DNA. C. Nuclease treatment degrades linker DNA, whichcan be up to 80 bp in length. This region of DNA is typically bound to linker histones (H1), which are involved in higher-level packing of the chromatin. Title: A. How can nucleosome core particles be isolated from chromatin? B. What molecular components were ... Post by: BallsDeep on Feb 3, 2018 Powerful stuff here, thanks
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