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Title: Which of the following statements about gel-transfer hybridization (or Southern blotting) is false? ... Post by: alenavar on Feb 3, 2018 Which of the following statements about gel-transfer hybridization (or Southern blotting) is false?
(a) This technique involves the transfer of DNA molecules from gel onto nitrocellulose paper or nylon paper. (b) In this technique, single-stranded DNA is separated by electrophoresis. (c) A labeled DNA probe binds to the DNA by hybridization. (d) The DNA that is separated on a gel is not labeled. Title: Which of the following statements about gel-transfer hybridization (or Southern blotting) is false? ... Post by: hellokristi4 on Feb 3, 2018 (b) During Southern blotting, double-stranded DNA is loaded onto the agarose gel. The DNA becomes denatured (and thus single-stranded) as it gets transferred by the alkali solution from the gel to the nitrocellulose or nylon sheet.
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