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Title: The gene for B-globin is a split gene, which means it contains introns. How would that affect a plan ... Post by: Revolver38 on Feb 4, 2018 The gene for B-globin is a split gene, which means it contains introns. How would that affect a plan to clone human B-globin in bacteria?
What will be an ideal response? Title: The gene for B-globin is a split gene, which means it contains introns. How would that affect a plan ... Post by: NILLABRO on Feb 4, 2018 Since bacteria do not process RNA or proteins the way we do, a split gene is more difficult to clone and express. The split gene can be cloned normally, but its expressed product will most likely be wrong. To compensate for this, the inserted DNA should be cDNA, which will not have the introns, since it was created from mRNA.
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