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Title: In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then ... Post by: shoppa on Feb 4, 2018 In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then neutralized before blotting. What is the primary function of the alkaline treatment?
a. It neutralizes any acid soluble impurities in the gel. b. It cleaves the DNA into smaller fragments to permit greater efficiency of transfer. c. It inactivates any restriction endonucleases that may be in the gel. d. It neutralizes any acidic phosphate groups that might prevent hybridization. e. It denatures the duplex DNA to single-stranded DNA (ssDNA). Title: In the Southern hybridization procedure, the gel after electrophoresis is treated with NaOH and then ... Post by: mickeymouse1 on Feb 4, 2018 Content hidden
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