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Title: The DNA concentration of purified DNA is important to molecular biology because? Post by: 0mg_Its_Sabby on Sep 24, 2014 How does estimating the concentration of purified DNA correlate with DNA purification?
So I just finished a molecular lab and we purified our given plasmid DNA which was E. Coli then we ran a gel and from that gel we had to estimate the concentration of our purified DNA. How does how well the purification went contribute to the concentration of the gel. I'm a little uncertain about this. Title: Re: The DNA concentration of purified DNA is important to molecular biology because? Post by: yukifangfang on Sep 26, 2014 If your purification is not good, basically, you will have RNA contamination. RNA is also nuclear acid that will give you band in the gel. As a result, your estimation will not be accurate enough. When you are looking at your gel, you might see a smear not a single band.
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