Title: Antibody Reconstitution Post by: knclayton on Feb 19, 2012 Hi All,
I have question regarding some antibodies I wish to reconstitute. The instruction form that came with the order states, "Reconstitute with 0.1 ml sterile distilled water (Final concentration=1mg/ml. Final buffer = 0.01 M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide)" So, does this mean I just use sterile distilled water, or am I somehow supposed to use PBS/BSA/etc., or is the PBS/BSA/etc. just part of the current makeup of the antibody vial that I have purchased? Thanks! Title: Re: Antibody Reconstitution Post by: robertling on Feb 19, 2012 I'm assuming the buffer comes with the kit and all you're supposed to do is add the water.
Good luck. Title: Re: Antibody Reconstitution Post by: knclayton on Feb 20, 2012 Thank you so much! Now here is a further question for that...
It says on the instructions below that: "storage condition: store at -20*C for one year. [...] When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4*C" So, how long would it be with water? Is PBS okay then too? Confused. Title: Re: Antibody Reconstitution Post by: duddy on Feb 20, 2012 Once you reconstitute it, it will expire in two weeks time, otherwise you'll need to start with a fresh batch.
|