|
Title: Describe, in general terms, the steps involved in the automated synthesis of the DNA sequence GCT. Post by: divijnanda on Feb 9, 2018 Describe, in general terms, the steps involved in the automated synthesis of the DNA sequence GCT.
Title: Describe, in general terms, the steps involved in the automated synthesis of the DNA sequence GCT. Post by: efslanwyk on Feb 9, 2018 Step 1
Attachment of 5'-DMT-N-benzoyl-2'-deoxyaguanosine to the polymer support by a 3' ester linkage. Step 2 Deprotection of the 5'-hydroxyl by cleavage of the DMT ether with dichloroacetic acid in CH2CL2. Step 3 Phosphite formation between the free 5'-hydroxyl of the polymer-bound N-protected 2'-deoxyguanosine and the phosphoramidite protected 5'-DMT-2'-deoxycytidine in the presence of tetrazole followed by oxidation to the phosphate triester with I2. Step 4 Repeat step 2 to deprotect the 5'-hydroxyl of the polymer-GC. Step 5 Repeat step 3 to form the phosphate triester between 5'-hydroxyl of 2'-deoxycytidine residue and phosphoramidite protected 5'-DMT-N-benzoyl-2'-deoxythymidine. Step 6 Cleave all protecting groups from the heterocyclic bases from the phosphates, as well as cleave the ester bond holding the DNA to the silica by treatment with aqueous ammonia. Title: Describe, in general terms, the steps involved in the automated synthesis of the DNA sequence GCT. Post by: divijnanda on Feb 9, 2018 Brilliant
|