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Title: Describe the procedure used for colony hybridization and why it is useful. Post by: colleen on Mar 24, 2012 Describe the procedure used for colony hybridization and why it is useful.
Title: Re: Describe the procedure used for colony hybridization and why it is useful. Post by: star on Apr 1, 2012 A plate containing several different colonies can be probed for a particular sequence of interest using colony hybridization. After replica plating (to save viable cells), one replica set of colonies are lysed and their nucleic acids are bound onto a filter paper. This paper is then subjected to a traditional Southern blot using a specific nucleic acid probe to observe the presence of a particular gene of interest.
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