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Biology-Related Homework Help Cell Biology Topic started by: colleen on Mar 24, 2012



Title: Why might transposon mutagenesis be favored over chemical mutagenesis?
Post by: colleen on Mar 24, 2012
Why might transposon mutagenesis be favored over chemical mutagenesis?


Title: Re: Why might transposon mutagenesis be favored over chemical mutagenesis?
Post by: bio_man on Mar 26, 2012
The use of transposons such as Tn5 and Tn10 is an excellent way to positively select for mutants using kanamycin or tetracycline (respectively) in a growth medium. This is very useful, especially when there is no other way to select for the newly generated mutants, because only the mutants containing the Tn-inserted antibiotic resistance marker will grow.