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Title: How can an open reading frame (ORF) be used to determine the location of a prote Post by: colleen on Apr 8, 2012 How can an open reading frame (ORF) be used to determine the location of a protein-encoding gene?
Title: Re: How can an open reading frame (ORF) be used to determine the location of a prote Post by: chemb on Apr 17, 2012 an ORF is often predicted by identifying a start codon, followed by a nucleotide sequence, and a terminating nonsense codon in the same reading frame. Once an ORF is predicted using computational (i.e., bioinformatics) methods, it then is possible for a geneticist to identify the mRNA transcript to confirm the ORF is a gene. From the ORF sequence, the amino acid sequence can be predicted, which aids in the biochemical characterization of the translated protein.
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