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Title: Using Dissociation Constant Conceptually? Post by: Shibe on Sep 11, 2021 Hello,
I’m completely stumped on how to use the Kd to gauge relative concentration of bound vs free protein. I was told something along these lines: \[K_d = \frac{( [A] \times [B] )}{[A \cdot B]}\] “If you have fixed amount of A, and is below Kd, there will be relatively little AB, and mostly free A” How does this make sense conceptually? I have been trying trying to draw the scenario out or prove it to myself mathematically but I am getting nowhere. Can anyone please help me? Title: Re: Using Dissociation Constant Conceptually? Post by: bio_man on Sep 11, 2021 Hi Shibe
I'm not sure what's necessarily being asked here. I found a resource online that might shine some light, but again, without an explanation of what those variables mean, I can't help further. https://ocw.mit.edu/courses/biology/7-51-graduate-biochemistry-fall-2001/lecture-notes/fa01lec07.pdf |