Biology Forums - Study Force

Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA).
Why a patient might test indeterminate?
    
Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
How would the results be affected if a negative control gave a positive result?
    
Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA).
How the direct and indirect ELISA are different?

Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
Explain where the epitope (antigenic determinant) is located
    
Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
Describe the importance of the washing steps in the direct antibody fluorescence test.

Activity 2: Comparing Samples with Ouchterlony Double Diffusion.
Why does the precipitin line form?
    
Activity 4: Western Blotting Technique.
Why the HIV Western blot is a more specific test than the indirect ELISA for HIV?
    
Activity 1: Using Direct Fluorescent Antibody Technique to Test for Chlamydia.
Describe how a positive result is detected in this serological test.

Activity 4: Western Blotting Technique.
Explain the procedure for a patient with an indeterminate HIV Western blot result.
    
Activity 2: Comparing Samples with Ouchterlony Double Diffusion.
Did you think human serum albumin and bovine serum albumin would have epitopes in common?
    

The patient might test indeterminate if they have not yet seroconverted or if they have not yet produced enough antibodies to yield a positive result.

Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA).
How the direct and indirect ELISA are different?.........

The Direct ELISA is used to detect antigens against a specific antibody bound in a test well
The Indirect ELISA is used to detect antibodies against an antigen bound in a test well.