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Title: how to find hill nnumber and min of subunit Post by: lisa142 on Apr 13, 2011 How do you find the hill number (nH) and minimum number of subunits in a hill plot graph? Can you give me like an example to show me it? Thnks!
Title: Re: how to find hill nnumber and min of subunit Post by: bio_man on Apr 13, 2011 Hey, could you provide some sort of example of what you're referring to.
Title: Re: how to find hill nnumber and min of subunit Post by: duddy on Apr 13, 2011 It's the slope...
(https://www.bio.cmu.edu/courses/03231/LecF04/Lec13/CoopHill.gif) In hill plots, the cooperative binding to multiple sites is nH < n. The graph shows Hill plots for the two examples below: (https://www.bio.cmu.edu/courses/03231/LecF04/Lec13/CoopSatn.gif) The noncooperative binding of ligand (blue) has a Hill coefficient, nH = 1.0. The cooperative binding of ligand (red) has a Hill coefficient, nH = 1.8 (the maximum slope on the Hill plot). Title: Re: how to find hill nnumber and min of subunit Post by: lisa142 on Apr 14, 2011 For example, from the table,
I find p50 to be about 71 but what would the nH be? Please really show me how to get this. I'm stuck getting the fact nH would be the max slope. I keep getting different numbers for each of the points I try. Need to know this for my test.... Title: Re: how to find hill nnumber and min of subunit Post by: duddy on Apr 14, 2011 For example, from the table, I find p50 to be about 71 but what would the nH be? Please really show me how to get this. I'm stuck getting the fact nH would be the max slope. I keep getting different numbers for each of the points I try. Need to know this for my test.... I thought you simply use the formula (y2 - y1) / (x2 - x1) = the slope. Since it's a linear relationship, you shouldn't get much deviation from one point to the next. |