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Title: Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduce Post by: Astatine on Apr 9, 2015 Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduced into a strain of E. coli. To better the odds of getting expression of the eukaryotic gene in this system, you need to make certain that
A) the gene has a eukaryotic promoter for transcription. B) the plasmid has a prokaryotic promoter for transcription. C) the gene and the plasmid were cut with different restriction enzymes. D) the gene product is toxic to the bacterial cell. E) mRNA is used for ligation directly into the vector. Title: Re: Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and intro Post by: virion on Apr 18, 2015 Content hidden
Title: Re: Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduce Post by: Astatine on Apr 20, 2015 Thanks for finding the answer, really appreciate it.
Title: Re: Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduce Post by: virion on Apr 21, 2015 No problem :D
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