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Title: In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and Post by: Astatine on Apr 9, 2015 In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and ligate the gene into a plasmid vector. However, even though all of the controls work, no transcription is obtained using your fragment. Working backward, you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene. It is possible that during restriction digestion
A) the termination signal was removed. B) the RNA polymerase became inactivated. C) primase could not bind. D) the promoter was removed. E) none of the above Title: Re: In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI Post by: virion on Apr 18, 2015 Content hidden
Title: Re: In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and Post by: Astatine on Apr 20, 2015 Glad I joined this site, thank you for your help.
Title: Re: In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and Post by: virion on Apr 21, 2015 It's a tricky question, glad I was able to deliver.
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