Title: ABC model of plant development + Sea urchin egg fertilization questions -help Post by: Rahpoo on Apr 21, 2015 Can someone help me answer these questions? I've been trying to figure them out for hours now :crying: Thanks!!
1) Flowers are composed of four organs, but in the ABC model of flower development biologists hypothesized that a total of only three genes (A, B, and C) are involved in specifying their identity. On what evidence was this hypothesis based? a) Only three classes of floral homeotic mutants have been found, each caused by a defect in a single gene. b) The A, B, and C genes trigger expression of other genes required for floral organ development. c) Genes are not involved in specifying the identities of floral organs that do not produce pollen or eggs. d) The four floral organs develop from only three whorls in the floral meristem. -i think the answer is b, but I'm not sure 2) (IMAGE ATTACHED) Based on what they knew about the fertilization envelope (a protective layer that forms around the egg when a sperm fuses with it), Steinhardt, Shatten, and their colleagues hypothesized that changes in the distribution of Ca2+ ions in sea urchin eggs are involved in the formation of the fertilization envelope. In sea urchin eggs (as in most eukaryotic cells) the concentration of Ca2+ ions is much higher in the endoplasmic reticulum (ER) than in the cytoplasm. To see how cytoplasmic Ca2+ concentration changes in the egg during fertilization, the researchers injected a Ca2+-specific fluorescent dye into the cytoplasm of unfertilized eggs. After adding sperm to the eggs, they observed the eggs with a fluorescence microscope. The following images show the changes in fluorescence that occurred after a single sperm entered the egg. The fluorescence of the dye increased with increasing cytoplasmic Ca2+ concentration. The green color indicates the region of the cell with the highest fluorescence at that point in time. Sort the labeled regions of the fertilized egg above based on the status of the ER Ca2+ channels and Ca2+ pumps in that region. Title: Re: ABC model of plant development + Sea urchin egg fertilization questions -help Post by: habiba on Apr 21, 2015 1) Flowers are composed of four organs, but in the ABC model of flower development biologists hypothesized that a total of only three genes (A, B, and C) are involved in specifying their identity. On what evidence was this hypothesis based? a) Only three classes of floral homeotic mutants have been found, each caused by a defect in a single gene. b) The A, B, and C genes trigger expression of other genes required for floral organ development. c) Genes are not involved in specifying the identities of floral organs that do not produce pollen or eggs. d) The four floral organs develop from only three whorls in the floral meristem. -i think the answer is b, but I'm not sure Each class of genes is required in two adjacent whorls. Class A genes are required in whorls 1 and 2, class B genes are required in whorls 2 and 3, and class C genes are required in whorls 3 and 4. Both class A and class B genes are required in whorl 2, and both class B and class C genes are required in whorl 3. The ABC model that summarizes these results is shown below. We'll talk in class about how this model has been tested. (http://biology.kenyon.edu/courses/biol114/Chap13/abc_model.gif) The A, B, and C genes are transcription factors. Different transcription factors are needed together to turn on a developmental gene program--such as A and B needed to initiate the program for petals. What turns on the different transcription factors in different cells? Induction and inhibition by one cell signalling to a neighboring cell. Repression of expression of A gene by C product, and of C gene by A product. Numerous other developmental gene products regulate or respond to A, B, and C. Title: Re: ABC model of plant development + Sea urchin egg fertilization questions -help Post by: Rahpoo on Apr 24, 2015 got it
1) A 2) Ca2+ channels closed, pumps operating slowly= a,c,f Ca2+ channels open= b,e,h Ca2+ channels closed, pumps operating rapidly= d,g |