|
Title: The Gal4 system quesyion Post by: Emma22 on Sep 12, 2015 Hello,
in terms of the Gal4 system, my understanding is that its a transcription factor that drives the expression of a gene (any gene x) downstream, hence we can identify the gene x (gene of interest). Please correct me if im wrong My question is, I am reading a paper about a gene that is involved in the circadian rhythem in fruit fly, and the author wanted to locate the loci of that gene by restricting the gene products to the Pigment-dispersing factor (pdf) at the ventral neurons by using Pdf-Gal4 . what do they mean by using Pdf-Gal4? thanks Title: Re: The Gal4 system quesyion Post by: jbouza92 on Sep 15, 2015 "Pdf-Gal4" is referring to the spot/location on the Gal4 gene that expression or repression of PDF occurs. Does it indicate that they form a binding domain?
either way, if you want to RESTRICT expression of the pdf at gal4, no binding of the promoter should occur. |