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Title: How does chemical degradation DNA sequencing (Maxam-Gilbert) differ from the dideoxy chain terminati Post by: tapey1996 on Oct 1, 2015 How does chemical degradation DNA sequencing (Maxam-Gilbert) differ from the dideoxy chain termination (Sanger) method?
A) A lower ratio of dideoxy:deoxy nucleotides is used. B) Ethidium bromide is used to visualize the DNA fragments instead of radioactive sulfur or phosphorus. C) Agarose gel is used for electrophoresis instead of high resolution polyacrylamide gel. D) "Rare cutter" restriction enzymes or nucleotide chemical modification are used to localize each base. Title: Re: How does chemical degradation DNA sequencing (Maxam-Gilbert) differ from the dideoxy chain termi Post by: de5iet on Oct 9, 2015 Content hidden
Title: Re: How does chemical degradation DNA sequencing (Maxam-Gilbert) differ from the dideoxy chain terminati Post by: tapey1996 on Oct 14, 2015 This answers it pretty well, thanks.
Title: Re: How does chemical degradation DNA sequencing (Maxam-Gilbert) differ from the dideoxy chain terminati Post by: de5iet on Oct 14, 2015 You're welcome :)
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