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Title: Does the enzyme Pepsin break down Casein? Post by: SMK.ANOOP on Sep 1, 2012 For a practical in school i was going to test Trypsin breaking down Casein, and thus turning milk powder clear. However, my school doesn't have and Trypsin, but they have Casein. I was wondering if anybody knew whether Pepsin also broke down Casein, thus making the milk go clear.
Title: Does the enzyme Pepsin break down Casein? Post by: Lexington on Sep 1, 2012 No you will need rennin to break down caesin
Title: Does the enzyme Pepsin break down Casein? Post by: buddhadog on Sep 2, 2012 should work:
Pepsin A, the major component, has a molecular weight of 35,000 daltons and an optimum pH of approximately 1.0 for substrates such as casein or hemoglobin if the substrate is native protein. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. watch for the pH!! |