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Title: Why is it necessary to digest a plasmid DNA and a genomic DNA with the same restriction enzyme? Post by: mirchem on Sep 11, 2012 why is it necessary?
Title: Why is it necessary to digest a plasmid DNA and a genomic DNA with the same restriction enzyme? Post by: daveys on Sep 11, 2012 I'm guessing you want to transform the genomic DNA with the plasmid DNA? If so, it's because you want the cuts to be mae in the same place, so both sequences are compatible.
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