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Title: After splitting Hematopoietic stem cells, how would one change the media? Post by: rkearl27 on Oct 3, 2012 The cells were washed with PBS, cleaved with trypsin, and media was added and the flask was then placed in an incubator. In order to change the media, do you just have to filter/suction out the previous media/solution and add another few ml's of media?
Title: After splitting Hematopoietic stem cells, how would one change the media? Post by: rjandmj82 on Oct 3, 2012 You can just pour off the old media and add the new since the cells will be sticking to the bottom of the tissue culture flask to form a layer.
If you want to split them up or scale up the flask it is best to use a centrifuge to separate the cells from the media. |